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Yes bacteriostatic water must be refrigerated at 28C immediately after the first needle puncture and used within 28 days

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K-means clustering of amplicon sequence variant data confirmed that 5-Amino-1MQ treatment generated a distinct cecal microbiome profile from all other treatment groups including diet-switched vehicle controls indicating that NNMT inhibition produces microbiome changes that are separable from those produced by dietary composition alone

Skorupski KA, Hammond GM, Irish AM, et al